As you're playing through Choo-Choo Charles, you'll immediately notice that you can attack Charles using the different weapons on the back of your train. As you progress, you'll find that Charles isn't the only thing you'll encounter that will attack you. In fact, masked characters can also attack you if they happen to see you.
If you're sneaky enough, you can sneak past them to avoid getting shot. But, if they do see you, they won't hesitate to shoot you down and kill you. You'll immediately respawn inside your train, but there's an alternative to just dying or running away fast enough for them to get tired of you and turn around. You can attack them back.
Related: How do you complete Swamp Swimmer in Choo-Choo Charles?
How do you arm yourself in Choo-Choo Charles?
Unfortunately, you can't arm yourself with any weapons in the game. But you can use the weapons attached to the back of your train. These weapons range from a Gatling gun to a missile launcher, making attacking anything that comes your way a breeze. You'll have to be quick, though, as these enemies can run really fast and have a high attack power when they land a shot. Considering this, you'll have to be quick when you have them follow you to your train just for you to hop on the back and shoot them down.
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